A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2.
Identifieur interne : 000245 ( Main/Exploration ); précédent : 000244; suivant : 000246A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2.
Auteurs : Cecy R. Xi [Australie] ; Arianna Di Fazio [Australie] ; Naveed Ahmed Nadvi [Australie] ; Karishma Patel [Australie] ; Michelle Sui Wen Xiang [Australie] ; Hui Emma Zhang [Australie] ; Chandrika Deshpande [Australie] ; Jason K K. Low [Australie] ; Xiaonan Trixie Wang [Australie] ; Yiqian Chen [Australie] ; Christopher L D. Mcmillan [Australie] ; Ariel Isaacs [Australie] ; Brenna Osborne [Australie] ; Ana Júlia Vieira De Ribeiro [Australie] ; Geoffrey W. Mccaughan [Australie] ; Joel P. Mackay [Australie] ; W Bret Church [Australie] ; Mark D. Gorrell [Australie]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2020.
Abstract
Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of the MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulphate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion-exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected by surface plasmon resonance or ELISA. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.
DOI: 10.3390/molecules25225392
PubMed: 33218025
PubMed Central: PMC7698748
Affiliations:
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Mccaughan</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>AW Morrow GE & Liver Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>AW Morrow GE & Liver Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050</wicri:regionArea><wicri:noRegion>NSW 2050</wicri:noRegion></affiliation></author><author><name sortKey="Mackay, Joel P" sort="Mackay, Joel P" uniqKey="Mackay J" first="Joel P" last="Mackay">Joel P. Mackay</name><affiliation wicri:level="4"><nlm:affiliation>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Church, W Bret" sort="Church, W Bret" uniqKey="Church W" first="W Bret" last="Church">W Bret Church</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Gorrell, Mark D" sort="Gorrell, Mark D" uniqKey="Gorrell M" first="Mark D" last="Gorrell">Mark D. Gorrell</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33218025</idno><idno type="pmid">33218025</idno><idno type="doi">10.3390/molecules25225392</idno><idno type="pmc">PMC7698748</idno><idno type="wicri:Area/Main/Corpus">000034</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000034</idno><idno type="wicri:Area/Main/Curation">000034</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000034</idno><idno type="wicri:Area/Main/Exploration">000034</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2.</title><author><name sortKey="Xi, Cecy R" sort="Xi, Cecy R" uniqKey="Xi C" first="Cecy R" last="Xi">Cecy R. Xi</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Di Fazio, Arianna" sort="Di Fazio, Arianna" uniqKey="Di Fazio A" first="Arianna" last="Di Fazio">Arianna Di Fazio</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Nadvi, Naveed Ahmed" sort="Nadvi, Naveed Ahmed" uniqKey="Nadvi N" first="Naveed Ahmed" last="Nadvi">Naveed Ahmed Nadvi</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Research Portfolio Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research Portfolio Core Research Facilities, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Patel, Karishma" sort="Patel, Karishma" uniqKey="Patel K" first="Karishma" last="Patel">Karishma Patel</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Xiang, Michelle Sui Wen" sort="Xiang, Michelle Sui Wen" uniqKey="Xiang M" first="Michelle Sui Wen" last="Xiang">Michelle Sui Wen Xiang</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Zhang, Hui Emma" sort="Zhang, Hui Emma" uniqKey="Zhang H" first="Hui Emma" last="Zhang">Hui Emma Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Deshpande, Chandrika" sort="Deshpande, Chandrika" uniqKey="Deshpande C" first="Chandrika" last="Deshpande">Chandrika Deshpande</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Low, Jason K K" sort="Low, Jason K K" uniqKey="Low J" first="Jason K K" last="Low">Jason K K. Low</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Wang, Xiaonan Trixie" sort="Wang, Xiaonan Trixie" uniqKey="Wang X" first="Xiaonan Trixie" last="Wang">Xiaonan Trixie Wang</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Chen, Yiqian" sort="Chen, Yiqian" uniqKey="Chen Y" first="Yiqian" last="Chen">Yiqian Chen</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Mcmillan, Christopher L D" sort="Mcmillan, Christopher L D" uniqKey="Mcmillan C" first="Christopher L D" last="Mcmillan">Christopher L D. Mcmillan</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072</wicri:regionArea><wicri:noRegion>QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Isaacs, Ariel" sort="Isaacs, Ariel" uniqKey="Isaacs A" first="Ariel" last="Isaacs">Ariel Isaacs</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072</wicri:regionArea><wicri:noRegion>QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Osborne, Brenna" sort="Osborne, Brenna" uniqKey="Osborne B" first="Brenna" last="Osborne">Brenna Osborne</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Vieira De Ribeiro, Ana Julia" sort="Vieira De Ribeiro, Ana Julia" uniqKey="Vieira De Ribeiro A" first="Ana Júlia" last="Vieira De Ribeiro">Ana Júlia Vieira De Ribeiro</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Mccaughan, Geoffrey W" sort="Mccaughan, Geoffrey W" uniqKey="Mccaughan G" first="Geoffrey W" last="Mccaughan">Geoffrey W. Mccaughan</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>AW Morrow GE & Liver Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>AW Morrow GE & Liver Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050</wicri:regionArea><wicri:noRegion>NSW 2050</wicri:noRegion></affiliation></author><author><name sortKey="Mackay, Joel P" sort="Mackay, Joel P" uniqKey="Mackay J" first="Joel P" last="Mackay">Joel P. Mackay</name><affiliation wicri:level="4"><nlm:affiliation>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Church, W Bret" sort="Church, W Bret" uniqKey="Church W" first="W Bret" last="Church">W Bret Church</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Gorrell, Mark D" sort="Gorrell, Mark D" uniqKey="Gorrell M" first="Mark D" last="Gorrell">Mark D. Gorrell</name><affiliation wicri:level="4"><nlm:affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of the MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulphate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion-exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected by surface plasmon resonance or ELISA. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33218025</PMID><DateRevised><Year>2020</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>22</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>18</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E5392</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules25225392</ELocationID><Abstract><AbstractText>Proteases catalyse irreversible posttranslational modifications that often alter a biological function of the substrate. The protease dipeptidyl peptidase 4 (DPP4) is a pharmacological target in type 2 diabetes therapy primarily because it inactivates glucagon-like protein-1. DPP4 also has roles in steatosis, insulin resistance, cancers and inflammatory and fibrotic diseases. In addition, DPP4 binds to the spike protein of the MERS virus, causing it to be the human cell surface receptor for that virus. DPP4 has been identified as a potential binding target of SARS-CoV-2 spike protein, so this question requires experimental investigation. Understanding protein structure and function requires reliable protocols for production and purification. We developed such strategies for baculovirus generated soluble recombinant human DPP4 (residues 29-766) produced in insect cells. Purification used differential ammonium sulphate precipitation, hydrophobic interaction chromatography, dye affinity chromatography in series with immobilised metal affinity chromatography, and ion-exchange chromatography. The binding affinities of DPP4 to the SARS-CoV-2 full-length spike protein and its receptor-binding domain (RBD) were measured using surface plasmon resonance and ELISA. This optimised DPP4 purification procedure yielded 1 to 1.8 mg of pure fully active soluble DPP4 protein per litre of insect cell culture with specific activity >30 U/mg, indicative of high purity. No specific binding between DPP4 and CoV-2 spike protein was detected by surface plasmon resonance or ELISA. In summary, a procedure for high purity high yield soluble human DPP4 was achieved and used to show that, unlike MERS, SARS-CoV-2 does not bind human DPP4.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xi</LastName><ForeName>Cecy R</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Di Fazio</LastName><ForeName>Arianna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nadvi</LastName><ForeName>Naveed Ahmed</ForeName><Initials>NA</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Portfolio Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Karishma</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0003-3034-3840</Identifier><AffiliationInfo><Affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Michelle Sui Wen</ForeName><Initials>MSW</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hui Emma</ForeName><Initials>HE</Initials><Identifier Source="ORCID">0000-0002-4699-4237</Identifier><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deshpande</LastName><ForeName>Chandrika</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0002-5557-519X</Identifier><AffiliationInfo><Affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Low</LastName><ForeName>Jason K K</ForeName><Initials>JKK</Initials><Identifier Source="ORCID">0000-0003-0862-0012</Identifier><AffiliationInfo><Affiliation>Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiaonan Trixie</ForeName><Initials>XT</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yiqian</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McMillan</LastName><ForeName>Christopher L D</ForeName><Initials>CLD</Initials><Identifier Source="ORCID">0000-0002-5316-0986</Identifier><AffiliationInfo><Affiliation>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Isaacs</LastName><ForeName>Ariel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Osborne</LastName><ForeName>Brenna</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-0197-3010</Identifier><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vieira de Ribeiro</LastName><ForeName>Ana Júlia</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCaughan</LastName><ForeName>Geoffrey W</ForeName><Initials>GW</Initials><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>AW Morrow GE & Liver Centre, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mackay</LastName><ForeName>Joel P</ForeName><Initials>JP</Initials><Identifier Source="ORCID">0000-0001-7508-8033</Identifier><AffiliationInfo><Affiliation>Drug Discovery, Sydney Analytical, Core Research Facilities, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Church</LastName><ForeName>W Bret</ForeName><Initials>WB</Initials><Identifier Source="ORCID">0000-0002-8033-5518</Identifier><AffiliationInfo><Affiliation>Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gorrell</LastName><ForeName>Mark D</ForeName><Initials>MD</Initials><Identifier Source="ORCID">0000-0002-0528-2604</Identifier><AffiliationInfo><Affiliation>Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1105238</GrantID><Agency>National Health and Medical Research Council</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Covid-19</Keyword><Keyword MajorTopicYN="N">DPP4</Keyword><Keyword MajorTopicYN="N">protease</Keyword><Keyword MajorTopicYN="N">recombinant protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>11</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>21</Day><Hour>1</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33218025</ArticleId><ArticleId IdType="pii">molecules25225392</ArticleId><ArticleId IdType="doi">10.3390/molecules25225392</ArticleId><ArticleId IdType="pmc">PMC7698748</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Protein Sci. 2004 Feb;13(2):412-21</Citation><ArticleIdList><ArticleId IdType="pubmed">14718659</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Sci (Lond). 2005 Apr;108(4):277-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15584901</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Protoc Microbiol. 2020 Jun;57(1):e100</Citation><ArticleIdList><ArticleId IdType="pubmed">32302069</ArticleId></ArticleIdList></Reference><Reference><Citation>Regul Pept. 1993 Dec 10;49(2):133-44</Citation><ArticleIdList><ArticleId IdType="pubmed">7907802</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2020 Oct 30;21(21):</Citation><ArticleIdList><ArticleId IdType="pubmed">33143089</ArticleId></ArticleIdList></Reference><Reference><Citation>MethodsX. 2015 Oct 13;2:379-84</Citation><ArticleIdList><ArticleId IdType="pubmed">26587388</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734</Citation><ArticleIdList><ArticleId IdType="pubmed">32376634</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Open Bio. 2013 Dec 08;4:43-54</Citation><ArticleIdList><ArticleId IdType="pubmed">24371721</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2004 Oct 8;279(41):43330-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15213224</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 2009;463:191-222</Citation><ArticleIdList><ArticleId IdType="pubmed">19892174</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2020 Jul 17;369(6501):330-333</Citation><ArticleIdList><ArticleId IdType="pubmed">32366695</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2003 Jan 28;42(3):694-701</Citation><ArticleIdList><ArticleId IdType="pubmed">12534281</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Appl Biochem. 2006 Jul;45(Pt 1):13-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16584386</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 Mar;579(7798):270-273</Citation><ArticleIdList><ArticleId IdType="pubmed">32015507</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta Gen Subj. 2020 Sep;1864(9):129636</Citation><ArticleIdList><ArticleId IdType="pubmed">32433934</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2020 Feb 22;395(10224):565-574</Citation><ArticleIdList><ArticleId IdType="pubmed">32007145</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2010 Dec;17(12):1486-91</Citation><ArticleIdList><ArticleId IdType="pubmed">21076402</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Metab. 2019 Feb 5;29(2):320-334.e5</Citation><ArticleIdList><ArticleId IdType="pubmed">30393019</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation><ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2020 Jul;26(7):1033-1036</Citation><ArticleIdList><ArticleId IdType="pubmed">32398876</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 1999 Dec;266(3):798-810</Citation><ArticleIdList><ArticleId IdType="pubmed">10583373</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2014 Jul;1844(7):1248-59</Citation><ArticleIdList><ArticleId IdType="pubmed">24717288</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Jul;88(14):7796-805</Citation><ArticleIdList><ArticleId IdType="pubmed">24789777</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Mol Med. 2020 Jul 26;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32713161</ArticleId></ArticleIdList></Reference><Reference><Citation>J Diabetes. 2020 Sep;12(9):649-658</Citation><ArticleIdList><ArticleId IdType="pubmed">32394639</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunol Cell Biol. 2017 May;95(5):443-453</Citation><ArticleIdList><ArticleId IdType="pubmed">27899813</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3082-6</Citation><ArticleIdList><ArticleId IdType="pubmed">7909158</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Sci. 2004 Jan;13(1):145-54</Citation><ArticleIdList><ArticleId IdType="pubmed">14691230</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2020 Jun;20(6):669-677</Citation><ArticleIdList><ArticleId IdType="pubmed">32240634</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Rheum Dis. 2018 Nov;21(11):1915-1923</Citation><ArticleIdList><ArticleId IdType="pubmed">27990763</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2018 Mar 29;555(7698):673-677</Citation><ArticleIdList><ArticleId IdType="pubmed">29562231</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 May;581(7807):221-224</Citation><ArticleIdList><ArticleId IdType="pubmed">32225175</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol Methods. 1996 Jan 16;189(1):99-105</Citation><ArticleIdList><ArticleId IdType="pubmed">8576585</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2014 Dec;471-473:49-53</Citation><ArticleIdList><ArticleId IdType="pubmed">25461530</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Microbiol. 2020 Apr;5(4):562-569</Citation><ArticleIdList><ArticleId IdType="pubmed">32094589</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Sci (Lond). 2009 Sep 28;118(1):31-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19780719</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2019 Jan 1;321(1):69-79</Citation><ArticleIdList><ArticleId IdType="pubmed">30418475</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 1997 Dec 1;186(11):1865-72</Citation><ArticleIdList><ArticleId IdType="pubmed">9382885</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1980 Jul 1;189(1):27-34</Citation><ArticleIdList><ArticleId IdType="pubmed">7458904</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Microbes Infect. 2020 Dec;9(1):601-604</Citation><ArticleIdList><ArticleId IdType="pubmed">32178593</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation><ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Expr Purif. 2002 Aug;25(3):527-32</Citation><ArticleIdList><ArticleId IdType="pubmed">12182835</ArticleId></ArticleIdList></Reference><Reference><Citation>Scand J Immunol. 2011 Feb;73(2):102-11</Citation><ArticleIdList><ArticleId IdType="pubmed">21198750</ArticleId></ArticleIdList></Reference><Reference><Citation>Pharmacol Ther. 2019 Jun;198:135-159</Citation><ArticleIdList><ArticleId IdType="pubmed">30822465</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2008 Mar 5;582(5):819-25</Citation><ArticleIdList><ArticleId IdType="pubmed">18275857</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 2000 Oct;267(20):6140-50</Citation><ArticleIdList><ArticleId IdType="pubmed">11012666</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1978 Jul 10;253(13):4617-23</Citation><ArticleIdList><ArticleId IdType="pubmed">659438</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Biochem Cell Biol. 2004 Jul;36(7):1281-96</Citation><ArticleIdList><ArticleId IdType="pubmed">15109572</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Med. 2019 Jul;8(8):3918-3927</Citation><ArticleIdList><ArticleId IdType="pubmed">31124302</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2020 Apr 16;181(2):271-280.e8</Citation><ArticleIdList><ArticleId IdType="pubmed">32142651</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Endocrinol (Lausanne). 2019 Feb 15;10:80</Citation><ArticleIdList><ArticleId IdType="pubmed">30828317</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Apr 15;280(15):14911-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15695814</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Biol. 2003 Jan;10(1):19-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12483204</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 May;581(7807):215-220</Citation><ArticleIdList><ArticleId IdType="pubmed">32225176</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 1994 Mar;24(3):566-70</Citation><ArticleIdList><ArticleId IdType="pubmed">7907293</ArticleId></ArticleIdList></Reference><Reference><Citation>Structure. 2003 Aug;11(8):947-59</Citation><ArticleIdList><ArticleId IdType="pubmed">12906826</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Xi, Cecy R" sort="Xi, Cecy R" uniqKey="Xi C" first="Cecy R" last="Xi">Cecy R. Xi</name></region><name sortKey="Chen, Yiqian" sort="Chen, Yiqian" uniqKey="Chen Y" first="Yiqian" last="Chen">Yiqian Chen</name><name sortKey="Church, W Bret" sort="Church, W Bret" uniqKey="Church W" first="W Bret" last="Church">W Bret Church</name><name sortKey="Deshpande, Chandrika" sort="Deshpande, Chandrika" uniqKey="Deshpande C" first="Chandrika" last="Deshpande">Chandrika Deshpande</name><name sortKey="Deshpande, Chandrika" sort="Deshpande, Chandrika" uniqKey="Deshpande C" first="Chandrika" last="Deshpande">Chandrika Deshpande</name><name sortKey="Di Fazio, Arianna" sort="Di Fazio, Arianna" uniqKey="Di Fazio A" first="Arianna" last="Di Fazio">Arianna Di Fazio</name><name sortKey="Gorrell, Mark D" sort="Gorrell, Mark D" uniqKey="Gorrell M" first="Mark D" last="Gorrell">Mark D. Gorrell</name><name sortKey="Isaacs, Ariel" sort="Isaacs, Ariel" uniqKey="Isaacs A" first="Ariel" last="Isaacs">Ariel Isaacs</name><name sortKey="Low, Jason K K" sort="Low, Jason K K" uniqKey="Low J" first="Jason K K" last="Low">Jason K K. Low</name><name sortKey="Mackay, Joel P" sort="Mackay, Joel P" uniqKey="Mackay J" first="Joel P" last="Mackay">Joel P. Mackay</name><name sortKey="Mccaughan, Geoffrey W" sort="Mccaughan, Geoffrey W" uniqKey="Mccaughan G" first="Geoffrey W" last="Mccaughan">Geoffrey W. Mccaughan</name><name sortKey="Mccaughan, Geoffrey W" sort="Mccaughan, Geoffrey W" uniqKey="Mccaughan G" first="Geoffrey W" last="Mccaughan">Geoffrey W. Mccaughan</name><name sortKey="Mcmillan, Christopher L D" sort="Mcmillan, Christopher L D" uniqKey="Mcmillan C" first="Christopher L D" last="Mcmillan">Christopher L D. Mcmillan</name><name sortKey="Nadvi, Naveed Ahmed" sort="Nadvi, Naveed Ahmed" uniqKey="Nadvi N" first="Naveed Ahmed" last="Nadvi">Naveed Ahmed Nadvi</name><name sortKey="Nadvi, Naveed Ahmed" sort="Nadvi, Naveed Ahmed" uniqKey="Nadvi N" first="Naveed Ahmed" last="Nadvi">Naveed Ahmed Nadvi</name><name sortKey="Osborne, Brenna" sort="Osborne, Brenna" uniqKey="Osborne B" first="Brenna" last="Osborne">Brenna Osborne</name><name sortKey="Patel, Karishma" sort="Patel, Karishma" uniqKey="Patel K" first="Karishma" last="Patel">Karishma Patel</name><name sortKey="Vieira De Ribeiro, Ana Julia" sort="Vieira De Ribeiro, Ana Julia" uniqKey="Vieira De Ribeiro A" first="Ana Júlia" last="Vieira De Ribeiro">Ana Júlia Vieira De Ribeiro</name><name sortKey="Wang, Xiaonan Trixie" sort="Wang, Xiaonan Trixie" uniqKey="Wang X" first="Xiaonan Trixie" last="Wang">Xiaonan Trixie Wang</name><name sortKey="Xiang, Michelle Sui Wen" sort="Xiang, Michelle Sui Wen" uniqKey="Xiang M" first="Michelle Sui Wen" last="Xiang">Michelle Sui Wen Xiang</name><name sortKey="Zhang, Hui Emma" sort="Zhang, Hui Emma" uniqKey="Zhang H" first="Hui Emma" last="Zhang">Hui Emma Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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